Immortalized Mouse Natural Killer Cells

A clonal derivative from the CD3-NK1.1+NK cells developed from cocultures of OP-9S/LMJSN and post-5-FU bone marrow MNCs, the Immortalized Mouse Natural Killer Cells (KIL C.2) are dependent on IL-7 for proliferation and survival. Without culturing the cells in IL-7 the cells will undergo apoptosis within 24 hours. The cells are positive for CD43, CD51, CD94, and NKG2D surface markers which are comparable to the parental cells. If cultured with IL-2, the cells terminally differentiate exhibiting large azurophilic granules, cytoplasmic vacuoles, ruffled cell membranes, upregulation in granzyme B, and eventually die off in culture. It is recommended for use in studies relating to natural killer cell development.